Cell-Free Regenerative Approaches for Improved Musculoskeletal Tissue Repair
Tuesday, February 4, 2020
11:00am – 12:00pm
310 Kelly Hall, Virginia Tech Campus
Postdoctoral Fellow in the McKay Orthopedic Surgery Research Laboratory
University of Pennsylvania
Musculoskeletal injuries due to disease, traumatic injury, or repetitive activity are major problems worldwide. Current surgical treatments to repair musculoskeletal tissues have a high risk of donor site morbidity, mechanical failure, and re-injury. Given these complications, tissue engineering (TE) has been heavily explored as an alternative strategy to overcome the many disadvantages associated with the conventional repair methods by using a combination of biocompatible materials, cells, and biologic factors to improve the healing response. While there has been an increasing interest on the use of cellular-based TE approaches for musculoskeletal tissue repair, my work aims to avoid possible complications associated with autologous and allogenic cell sources and simplify the regulatory pathway towards clinical application by synergistically complementing and improving the native healing environment without the use of implanted cells. The featured studies focus on the in vitro and in vivo evaluation of two independent three- dimensional systems to enhance (1) bone regeneration and (2) tendon repair. First, the development and characterization of a novel osteoinductive pre-vascularized scaffold composed of electrospun synthetic and collagen-based materials designed to promote early vascularization for significant bone loss will be discussed. This innovative scaffold promoted the ingrowth of bone tissue and vasculature in a mouse model without the addition of growth factors or cells. Next, the biological and mechanical implications of a biocompatible nanofibrous bilayer delivery system (BiLDS) for local and sustained delivery of ibuprofen to mitigate inflammation in a rat rotator cuff injury and repair model will be featured. The BiLDS reduced the in vivo pro-inflammatory response and improved tendon mechanics over time. This collection of work exploits the ability of these cell-free transformative technologies to provide physical and chemical cues for improved musculoskeletal tissue repair.
Dr. Brittany Taylor is a Postdoctoral Fellow in the McKay Orthopedic Surgery Research Laboratory at the University of Pennsylvania (Penn) working with Dr. Louis Soslowsky. As a recipient of the Penn Provost’s Postdoctoral Fellowship, her research focuses on the development of a drug delivery system as a therapeutic for rotator cuff tendinopathy and investigating the role of collagen type V on tendon healing and homeostasis. Brittany received her B.S. in Biomedical Engineering from the University of Virginia in 2010 and her Ph.D. in Biomedical Engineering from Rutgers University in 2016. Brittany is an author on numerous peer-reviewed journal and patent review articles, 3 book chapters, and a co-inventor on two patents. Brittany is also a Burroughs Wellcome Fund Postdoctoral Fellow and the principal investigator (PI) of a grant to investigate extracellular vesicles as a therapeutic and diagnostic for diseased and injured musculoskeletal tissues.